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Ubiquilin 1 Polyclonal antibody

Ubiquilin 1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22126-1-AP

Print datasheet

Synonyms

UBQLN1, PLIC 1, hPLIC-1, hPLIC1, hPLIC 1

验证数据展示

  • WB analysis using 22126-1-AP

    WB analysis using 22126-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 22126-1-AP

    IF Staining of HeLa using 22126-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Ubiquilin 1 antibody (22126-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inT-47D cells, Transfected HEK-293 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22126-1-AP targets Ubiquilin 1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Ubiquilin 1 fusion protein Ag17530 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquilin 1
Synonyms UBQLN1, PLIC 1, hPLIC-1, hPLIC1, hPLIC 1
Calculated Molecular Weight 589 aa, 63 kDa
Observed Molecular Weight 63-71 kDa
GenBank Accession NumberBC010066
Gene Symbol Ubiquilin 1
Gene ID (NCBI) 29979
RRIDAB_2879000
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UMX0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBQLN1, also known as DSK2 or Ubiquilin 1, is an ubiquitin-like proteinis contains a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation.Ubiquilin has also been shown to modulate accumulation of presenilin proteins, and is found in lesions associated with Alzheimer's and Parkinson's disease. Two transcript variants encoding different isoforms have been found for this gene. Higher levels of ubiquilin-1 in the brain decreased malformation of the APP molecule which plays a key role in triggering Alzheimers disease.Conversely, lower levels of ubiquilin-1 in the brain were associated with increased malformation of APP (PMID: 21852239). This antibody detects 70kD band of UBQLN1.

实验方案

Product Specific Protocols
WB protocol for Ubiquilin 1 antibody 22126-1-APDownload protocol
IF protocol for Ubiquilin 1 antibody 22126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols