验证数据展示
经过测试的应用
| Positive IF/ICC detected in | U-87 MG cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67822 targets VAMP2 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, Mouse, Rat, Pig, Rabbit |
| 免疫原 | VAMP2 fusion protein Ag17908 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG3 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | vesicle-associated membrane protein 2 (synaptobrevin 2) |
| 别名 | FLJ11460, SYB2, Synaptobrevin 2, VAMP 2, VAMP2 |
| 计算分子量 | 13 kDa |
| 观测分子量 | 19 kDa |
| GenBank蛋白编号 | BC002737 |
| 基因名称 | VAMP2 |
| Gene ID (NCBI) | 6844 |
| RRID | AB_3084379 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P63027 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VAMP2 (vesicle-associated membrane protein 2), also named as synaptobrevin 2, is a member of the SNARE (soluble NSF-attachment protein receptor) family proteins. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP2, with a molecular mass of 15-19 kDa, consists of a short N-terminal sequence, a SNARE motif, and a C-terminal transmembrane region. It is required for fast calcium-triggered synaptic vesicle fusion. VAMP2 forms a stable complex with STX1 (syntaxin 1) and SNAP25 (synaptosomal-associated protein 25) during synaptic vesicle fusion (PMID: 16793874). It also forms a distinct complex with synaptophysin. VAMP2 is expressed in nervous system and some non-neuronal tissues, such as skeletal muscle (PMID: 18570252).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 VAMP2 antibody CL488-67822 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |