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  • Featured Product
  • KD/KO Validated

VAPA Polyclonal antibody, PBS Only

VAPA Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15275-1-PBS

Print datasheet

Synonyms

VAMP-A, VAMP associated protein A, VAMP A, hVAP 33, 33 kDa VAMP-associated protein

验证数据展示

  • WB analysis using 15275-1-AP (same clone as 15275-1-PBS)

    WB analysis using 15275-1-AP (same clone as 15275-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15275-1-AP (VAPA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 15275-1-AP (same clone as 15275-1-PBS)

    IHC staining of human colon cancer using 15275-1-AP (same clone as 15275-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 15275-1-AP (same clone as 15275-1-PBS)

    IHC staining of human colon cancer using 15275-1-AP (same clone as 15275-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 15275-1-AP (same clone as 15275-1-PBS)

    IP experiment of HEK-293 using 15275-1-AP (same clone as 15275-1-PBS)

    IP result of anti-VAPA (IP:15275-1-AP, 3ug; Detection:15275-1-AP 1:1000) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 15275-1-AP (same clone as 15275-1-PBS)

    IF Staining of HepG2 using 15275-1-AP (same clone as 15275-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAPA antibody (15275-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 15275-1-AP (same clone as 15275-1-PBS)

    IF Staining of HepG2 using 15275-1-AP (same clone as 15275-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAPA antibody (15275-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

产品信息

15275-1-PBS targets VAPA in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen VAPA fusion protein Ag7393 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
Synonyms VAMP-A, VAMP associated protein A, VAMP A, hVAP 33, 33 kDa VAMP-associated protein
Calculated Molecular Weight 28 kDa
Observed Molecular Weight28-30 kDa
GenBank Accession NumberBC002992
Gene Symbol VAPA
Gene ID (NCBI) 9218
RRIDAB_2256991
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P0L0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.