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VAPA Polyclonal antibody

VAPA Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 15275-1-AP

Print datasheet

Synonyms

33 kDa VAMP associated protein, hVAP 33, VAMP A, VAMP associated protein A, VAP 33, VAP A, VAP33, VAPA



经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, mouse skeletal muscle tissue, human kidney tissue, human brain tissue, mouse kidney tissue, mouse liver tissue, rat liver tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, mouse heart tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15275-1-AP targets VAPA in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications CoIP, IF, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Immunogen VAPA fusion protein Ag7393 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
Synonyms 33 kDa VAMP associated protein, hVAP 33, VAMP A, VAMP associated protein A, VAP 33, VAP A, VAP33, VAPA
Calculated Molecular Weight 28 kDa
Observed Molecular Weight28-30 kDa
GenBank Accession NumberBC002992
Gene Symbol VAPA
Gene ID (NCBI) 9218
RRIDAB_2256991
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P0L0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.

实验方案

Product Specific Protocols
WB protocol for VAPA antibody 15275-1-APDownload protocol
IHC protocol for VAPA antibody 15275-1-APDownload protocol
IF protocol for VAPA antibody 15275-1-APDownload protocol
IP protocol for VAPA antibody 15275-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanCOIP

Mol Cell

The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation.

Authors - Yan G Zhao
monkeyWB

Mol Cell

MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes.

Authors - Christophe A C Freyre
mouseWB

J Clin Invest

Upregulation of Rubicon promotes autosis during myocardial ischemia/reperfusion injury.

Authors - Jihoon Nah

EMBO J

A selective ER-phagy exerts procollagen quality control via a Calnexin-FAM134B complex.

Authors - Alison Forrester
humanWB

EMBO J

CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.

Authors - Thaddaeus Mutugi Nthiga
monkeyWB, CoIP,IF

Curr Biol

The ER Contact Proteins VAPA/B Interact with Multiple Autophagy Proteins to Modulate Autophagosome Biogenesis.

Authors - Yan G Zhao
  • KD Validated