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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated VAPA Polyclonal antibody

VAPA Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15275

Print datasheet

Synonyms

VAMP-A, VAMP associated protein A, VAMP A, hVAP 33, 33 kDa VAMP-associated protein

验证数据展示

  • IF Staining of HepG2 using CL488-15275

    IF Staining of HepG2 using CL488-15275

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 VAPA antibody (CL488-15275) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HepG2 using CL488-15275

    FC experiment of HepG2 using CL488-15275

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 VAPA Polyclonal Antibody (CL488-15275)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 15275-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15275 targets VAPA in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen VAPA fusion protein Ag7393 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
Synonyms VAMP-A, VAMP associated protein A, VAMP A, hVAP 33, 33 kDa VAMP-associated protein
Calculated Molecular Weight 28 kDa
Observed Molecular Weight28-30 kDa
GenBank Accession NumberBC002992
Gene Symbol VAPA
Gene ID (NCBI) 9218
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P0L0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 VAPA antibody CL488-15275Download protocol
FC protocol for CL Plus 488 VAPA antibody CL488-15275Download protocol
Standard Protocols
Click here to view our Standard Protocols