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VAPA Monoclonal antibody

VAPA Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F10F9

Cat no : 67845-1-Ig

Print datasheet

Synonyms

33 kDa VAMP associated protein, hVAP 33, VAMP A, VAMP associated protein A, VAP 33, VAP A, VAP33, VAPA

验证数据展示

  • WB analysis using 67845-1-Ig

    WB analysis using 67845-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67845-1-Ig (VAPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IHC staining of human liver cancer using 67845-1-Ig

    IHC staining of human liver cancer using 67845-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67845-1-Ig (VAPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67845-1-Ig

    IHC staining of human liver cancer using 67845-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67845-1-Ig (VAPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, Neuro-2a cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67845-1-Ig targets VAPA in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen VAPA fusion protein Ag7819 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
Synonyms 33 kDa VAMP associated protein, hVAP 33, VAMP A, VAMP associated protein A, VAP 33, VAP A, VAP33, VAPA
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC002992
Gene Symbol VAPA
Gene ID (NCBI) 9218
RRIDAB_2918607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9P0L0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a 33-kDa, ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.

实验方案

Product Specific Protocols
WB protocol for VAPA antibody 67845-1-IgDownload protocol
IHC protocol for VAPA antibody 67845-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols