验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, Neuro-2a cells |
Positive IHC detected in | human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67845-1-Ig targets VAPA in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | Human, Mouse, Rat |
免疫原 | VAPA fusion protein Ag7819 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa |
别名 | 33 kDa VAMP associated protein, hVAP 33, VAMP A, VAMP associated protein A, VAP 33, VAP A, VAP33, VAPA |
计算分子量 | 28 kDa |
观测分子量 | 33 kDa |
GenBank蛋白编号 | BC002992 |
基因名称 | VAPA |
Gene ID (NCBI) | 9218 |
RRID | AB_2918607 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q9P0L0 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a 33-kDa, ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for VAPA antibody 67845-1-Ig | Download protocol |
IHC protocol for VAPA antibody 67845-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |