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验证数据展示

  • FC experiment of HepG2 using CL488-67845

    FC experiment of HepG2 using CL488-67845

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human VAPA (CL488-67845, Clone:1F10F9) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated VAPA Monoclonal antibody

VAPA Monoclonal Antibody for FC (Intra)
Cat No. CL488-67845

产品说明书

CloneNo. 1F10F9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

VAMP associated protein A, VAMP A, hVAP 33, 33 kDa VAMP-associated protein, 33 kDa VAMP associated protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67845-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67845 targets VAPA in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 VAPA fusion protein Ag7819 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
别名 VAMP associated protein A, VAMP A, hVAP 33, 33 kDa VAMP-associated protein, 33 kDa VAMP associated protein
计算分子量 28 kDa
观测分子量 33 kDa
GenBank蛋白编号BC002992
基因名称 VAPA
Gene ID (NCBI) 9218
RRIDAB_2923801
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9P0L0
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a 33-kDa, ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 VAPA antibody CL488-67845Download protocol
Standard Protocols
Click here to view our Standard Protocols