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  • Featured Product
  • KD/KO Validated

VARS2 Polyclonal antibody, PBS Only

VARS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15776-1-PBS

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Synonyms

验证数据展示

  • IHC staining of human liver cancer using 15776-1-AP (same clone as 15776-1-PBS)

    IHC staining of human liver cancer using 15776-1-AP (same clone as 15776-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 15776-1-AP (same clone as 15776-1-PBS)

    IHC staining of human liver cancer using 15776-1-AP (same clone as 15776-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

  • WB analysis using 15776-1-AP (same clone as 15776-1-PBS)

    WB analysis using 15776-1-AP (same clone as 15776-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 15776-1-AP (same clone as 15776-1-PBS)

    IP experiment of HeLa using 15776-1-AP (same clone as 15776-1-PBS)

    IP result of anti-VARS2 (IP:15776-1-AP, 4ug; Detection:15776-1-AP 1:500) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 15776-1-AP (same clone as 15776-1-PBS)

    IF Staining of HeLa using 15776-1-AP (same clone as 15776-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15776-1-AP (VARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

产品信息

15776-1-PBS targets VARS2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human
Immunogen VARS2 fusion protein Ag8460 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name valyl-tRNA synthetase 2, mitochondrial (putative)
Synonyms
Calculated Molecular Weight 119 kDa, 57 kDa
Observed Molecular Weight 118 kDa
GenBank Accession NumberBC008844
Gene Symbol VARS2
Gene ID (NCBI) 57176
RRIDAB_2272699
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5ST30
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VARS2(Valine--tRNA ligase, mitochondrial) is also named as KIAA1885, VARS2L, VARSL and belongs to the class-I aminoacyl-tRNA synthetase family. The deduced 993-amino acid protein has an N-terminal mitochondrial targeting signal with a predicted cleavage site after residue 29. VARS2 has characteristics of a class I mitochondrial aminoacyl-tRNA synthetase, including a classical Rossmann fold.