• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

VASH2 Monoclonal antibody

VASH2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A11D10

Cat no : 67753-1-Ig

Print datasheet

Synonyms

FLJ12505, RP11 275G3.1, VASH2, VASHL, vasohibin 2, Vasohibin like protein

验证数据展示

  • WB analysis using 67753-1-Ig

    WB analysis using 67753-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67753-1-Ig (VASH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of human skin cancer using 67753-1-Ig

    IHC staining of human skin cancer using 67753-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inSKOV-3 cells, BGC-823 cells, L02 cells, HepG2 cells, HUVEC cells, HSC-T6 cells, NIH/3T3 cells, SGC-7901 cells, MKN-45 cells
Positive IHC detected inhuman skin cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67753-1-Ig targets VASH2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen VASH2 fusion protein Ag29985 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vasohibin 2
Synonyms FLJ12505, RP11 275G3.1, VASH2, VASHL, vasohibin 2, Vasohibin like protein
Calculated Molecular Weight 355 aa, 40 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC028194
Gene Symbol VASH2
Gene ID (NCBI) 79805
RRIDAB_2918521
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ86V25
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VASH2, also named as vasohibin 2, is an important pro-angiogenesis factor in solid tumor. It has been reported that VASH2 is expressed in mononuclear cells mobilized from bone marrow to promote angiogenesis. VASH2 also plays a key role in axon formation. VASH2 is localized as a nuclear type(with 311 amino acid residues) and cytoplasmic type (with 355 amino acid residues and low abundance). Cytoplasmic VASH2 is associated with carcinoma angiogenesis, while nuclear VASH2 may be associated with cell proliferation. 67753-1-Ig antibody recognizes both the nuclear and cytoplasmic isoforms. (PMID:23615928; 19204325; 31235911; 26177649)

实验方案

Product Specific Protocols
WB protocol for VASH2 antibody 67753-1-IgDownload protocol
IHC protocol for VASH2 antibody 67753-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols