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Mouse VCAM-1/CD106 Recombinant antibody, PBS Only (Capture)

VCAM-1/CD106 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240597B2

Cat no : 83719-1-PBS

Print datasheet

Synonyms

CD106, VCAM-1, Vcam1, Vcam 1, 240597B2

验证数据展示

  • WB analysis of 3T3-L1 using 83719-1-RR (same clone as 83719-1-PBS)

    WB analysis of 3T3-L1 using 83719-1-RR (same clone as 83719-1-PBS)

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 83719-1-RR (VCAM-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83719-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00701-4

    Sandwich ELISA standard curve of MP00701-4

    Sandwich ELISA standard curve of MP00701-4, Mouse VCAM-1/CD106 Recombinant Matched Antibody Pair - PBS only. 83719-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0639. 83719-3-PBS was HRP conjugated as the detection antibody. Range: 46.88-3000 pg/mL.

  • Sample test of MP00701-4

    Sample test of MP00701-4

    Urine of eight mice was measured. The VCAM-1 concentration of detected samples was determined to be 71.97 ng/mL with a range of 8.65-105.58 ng/mL.

  • Sample test of MP00701-4

    Sample test of MP00701-4

    Mouse lung, cut into 1-2 mm pieces, was cultured for 3 days in 25-30 mL of RPMI supplemented with 10% fetal bovine serum and unstimulated or stimulated with 10 μg/mL ConA. The mean VCAM-1 concentration was determined to be 14.2 ng/mL in unstimulated mouse lung superantant, 20.9 ng/mL in ConA stimulated mouse lung superantant.

  • Sample test of MP00701-4

    Sample test of MP00701-4

    Serum of eight mice was measured. The VCAM-1 concentration of detected samples was determined to be 572.76 ng/mL with a range of 483.18-844.96 ng/mL.

  • Cytometric bead array standard curve of MP00701-1

    Cytometric bead array standard curve of MP00701-1

    Cytometric bead array standard curve of MP00701-1, MOUSE VCAM-1/CD106 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83719-1-PBS. Detection antibody: 83719-4-PBS. Standard: Eg0639. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83719-1-RR

    Affinity and Kinetic Characterization of 83719-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83719-1-RR against Mouse VCAM-1 were performed. The affinity constant is 0.383 nM.

产品信息

83719-1-PBS targets VCAM-1/CD106 as part of a matched antibody pair:

MP00701-1: 83719-1-PBS capture and 83719-4-PBS detection (validated in Cytometric bead array)

MP00701-4: 83719-1-PBS capture and 83719-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name vascular cell adhesion molecule 1
Synonyms CD106, VCAM-1, Vcam1, Vcam 1, 240597B2
Calculated Molecular Weight81 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberCAA47989.1
Gene Symbol VCAM-1
Gene ID (NCBI) 22329
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29533
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).