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验证数据展示

  • WB analysis of 3T3-L1 using 83719-1-RR

    WB analysis of 3T3-L1 using 83719-1-RR

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 83719-1-RR (VCAM-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 83719-1-RR

    IHC staining of mouse spleen using 83719-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83719-1-RR (VCAM-1/CD106 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 83719-1-RR

    IHC staining of mouse spleen using 83719-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83719-1-RR (VCAM-1/CD106 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83719-1-RR

    Affinity and Kinetic Characterization of 83719-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83719-1-RR against Mouse VCAM-1 were performed. The affinity constant is 0.383 nM.

VCAM-1/CD106 Recombinant antibody

VCAM-1/CD106 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83719-1-RR

产品说明书

CloneNo. 240597B2

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

CD106, VCAM-1, Vcam1, Vcam 1, 240597B2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected in3T3-L1 cells
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83719-1-RR targets VCAM-1/CD106 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vascular cell adhesion molecule 1
别名 CD106, VCAM-1, Vcam1, Vcam 1, 240597B2
计算分子量81 kDa
观测分子量90 kDa
GenBank蛋白编号CAA47989.1
基因名称 VCAM-1
Gene ID (NCBI) 22329
RRIDAB_3671321
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP29533
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

实验方案

Product Specific Protocols
WB protocol for VCAM-1/CD106 antibody 83719-1-RRDownload protocol
IHC protocol for VCAM-1/CD106 antibody 83719-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols