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验证数据展示

  • WB analysis using 84225-2-RR (same clone as 84225-2-PBS)

    WB analysis using 84225-2-RR (same clone as 84225-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84225-2-RR (VDAC2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84225-2-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84225-2-RR (same clone as 84225-2-PBS)

    FC experiment of HepG2 using 84225-2-RR (same clone as 84225-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Vdac2 Recombinant Antibody (84225-2-RR, Clone:241569G11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84225-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01162-1

    Cytometric bead array standard curve of MP01162-1

    Cytometric bead array standard curve of MP01162-1, VDAC2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84225-2-PBS. Detection antibody: 84225-1-PBS. Standard: Ag2266. Range: 1.563-200 ng/mL.

  • Affinity and Kinetic Characterization of 84225-2-RR

    Affinity and Kinetic Characterization of 84225-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84225-2-RR against Human VDAC2 were performed. The affinity constant is 0.48 nM.

VDAC2 Recombinant antibody, PBS Only (Capture)

VDAC2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 84225-2-PBS

产品说明书

CloneNo. 241569G11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), Cytometric bead array, Indirect ELISA

别名

VDAC-2, VDAC 2, hVDAC2, FLJ23841, 241569G11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy VDAC2 Ready-To-Use IHC Kit. VDAC2 primary antibody included.

产品信息

84225-2-PBS targets VDAC2 as part of a matched antibody pair:

MP01162-1: 84225-2-PBS capture and 84225-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 VDAC2 fusion protein Ag2266 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 voltage-dependent anion channel 2
别名 VDAC-2, VDAC 2, hVDAC2, FLJ23841, 241569G11
计算分子量 294 aa, 32 kDa
观测分子量 31-33 kDa
GenBank蛋白编号BC000165
基因名称 VDAC2
Gene ID (NCBI) 7417
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP45880
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VDACs (Voltage Dependent Anion selective Channels), also known as mitochondrial porins, are a family of pore-forming proteins discovered in the mitochondrial outer membrane. Mammals show a conserved genetic organization of the VDAC genes. It's reported that the amount of VDAC transcripts in liver is usually lower than in the other tissues. VDAC2 and especially VDAC3 are highly expressed in testis, while mouse VDAC1 is poorly expressed in this tissue. (PMID: 22020053)