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VDAC2 Recombinant antibody

VDAC2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241569G11

Cat no : 84225-2-RR

Print datasheet

Synonyms

VDAC-2, VDAC 2, hVDAC2, FLJ23841, 241569G11

验证数据展示

  • WB analysis using 84225-2-RR

    WB analysis using 84225-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84225-2-RR (VDAC2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 84225-2-RR

    FC experiment of HepG2 using 84225-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Vdac2 Recombinant Antibody (84225-2-RR, Clone:241569G11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84225-2-RR

    Affinity and Kinetic Characterization of 84225-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84225-2-RR against Human VDAC2 were performed. The affinity constant is 0.48 nM.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HuH-7 cells, L02 cells, mouse brain tissue, mouse heart tissue, rat brain tissue
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy VDAC2 Ready-To-Use IHC Kit. VDAC2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84225-2-RR targets VDAC2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen VDAC2 fusion protein Ag2266 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name voltage-dependent anion channel 2
Synonyms VDAC-2, VDAC 2, hVDAC2, FLJ23841, 241569G11
Calculated Molecular Weight 294 aa, 32 kDa
Observed Molecular Weight 31-33 kDa
GenBank Accession NumberBC000165
Gene Symbol VDAC2
Gene ID (NCBI) 7417
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP45880
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VDACs (Voltage Dependent Anion selective Channels), also known as mitochondrial porins, are a family of pore-forming proteins discovered in the mitochondrial outer membrane. Mammals show a conserved genetic organization of the VDAC genes. It's reported that the amount of VDAC transcripts in liver is usually lower than in the other tissues. VDAC2 and especially VDAC3 are highly expressed in testis, while mouse VDAC1 is poorly expressed in this tissue. (PMID: 22020053)

实验方案

Product Specific Protocols
WB protocol for VDAC2 antibody 84225-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols