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验证数据展示

  • WB analysis using 84225-2-RR

    WB analysis using 84225-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84225-2-RR (VDAC2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 84225-2-RR

    FC experiment of HepG2 using 84225-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Vdac2 Recombinant Antibody (84225-2-RR, Clone:241569G11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84225-2-RR

    Affinity and Kinetic Characterization of 84225-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84225-2-RR against Human VDAC2 were performed. The affinity constant is 0.48 nM.

VDAC2 Recombinant antibody

VDAC2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84225-2-RR

产品说明书

CloneNo. 241569G11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

VDAC-2, VDAC 2, hVDAC2, FLJ23841, 241569G11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HuH-7 cells, L02 cells, mouse brain tissue, mouse heart tissue, rat brain tissue
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy VDAC2 Ready-To-Use IHC Kit. VDAC2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84225-2-RR targets VDAC2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 VDAC2 fusion protein Ag2266 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 voltage-dependent anion channel 2
别名 VDAC-2, VDAC 2, hVDAC2, FLJ23841, 241569G11
计算分子量 294 aa, 32 kDa
观测分子量 31-33 kDa
GenBank蛋白编号BC000165
基因名称 VDAC2
Gene ID (NCBI) 7417
RRIDAB_3671778
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP45880
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VDACs (Voltage Dependent Anion selective Channels), also known as mitochondrial porins, are a family of pore-forming proteins discovered in the mitochondrial outer membrane. Mammals show a conserved genetic organization of the VDAC genes. It's reported that the amount of VDAC transcripts in liver is usually lower than in the other tissues. VDAC2 and especially VDAC3 are highly expressed in testis, while mouse VDAC1 is poorly expressed in this tissue. (PMID: 22020053)

实验方案

Product Specific Protocols
WB protocol for VDAC2 antibody 84225-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols