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验证数据展示

  • WB analysis using 83766-2-RR (same clone as 83766-2-PBS)

    WB analysis using 83766-2-RR (same clone as 83766-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83766-2-RR (VE-cadherin/CD144 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83766-2-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 83766-2-RR (same clone as 83766-2-PBS)

    IHC staining of human placenta using 83766-2-RR (same clone as 83766-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83766-2-RR (VE-cadherin antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83766-2-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 83766-2-RR (same clone as 83766-2-PBS)

    IHC staining of human placenta using 83766-2-RR (same clone as 83766-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83766-2-RR (VE-cadherin/CD144 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83766-2-PBS in a different storage buffer formulation.

  • IF Staining of HUVEC using 83766-2-RR (same clone as 83766-2-PBS)

    IF Staining of HUVEC using 83766-2-RR (same clone as 83766-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using VE-cadherin antibody (83766-2-RR, Clone: 240755D8 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83766-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00752-1

    Cytometric bead array standard curve of MP00752-1

    Cytometric bead array standard curve of MP00752-1, VE-cadherin/CD144 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83766-2-PBS. Detection antibody: 83766-4-PBS. Standard: Eg1200. Range: 0.78-100 ng/mL.

  • Cytometric bead array standard curve of MP00752-2

    Cytometric bead array standard curve of MP00752-2

    Cytometric bead array standard curve of MP00752-2, VE-cadherin/CD144 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83766-2-PBS. Detection antibody: 83766-3-PBS. Standard: Eg1200. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83766-2-RR

    Affinity and Kinetic Characterization of 83766-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83766-2-RR against Human VE-cadherin were performed. The affinity constant is 0.273 nM.

VE-cadherin/CD144 Recombinant antibody, PBS Only (Capture)

VE-cadherin/CD144 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83766-2-PBS

产品说明书

CloneNo. 240755D8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83766-2-PBS targets VE-cadherin/CD144 as part of a matched antibody pair:

MP00752-1: 83766-2-PBS capture and 83766-4-PBS detection (validated in Cytometric bead array)

MP00752-2: 83766-2-PBS capture and 83766-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cadherin 5, type 2 (vascular endothelium)
别名 VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen
计算分子量88 kDa
观测分子量130 kDa, 100 kDa
GenBank蛋白编号NM_001795.5
基因名称 VE-cadherin
Gene ID (NCBI) 1003
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP33151-1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).