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Rat VEGF 164 Recombinant antibody, PBS Only (Capture/Detector)

VEGF 164 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240573B3

Cat no : 83610-2-PBS

Print datasheet

Synonyms

Vegfa, VEGF164, Vegf, 240573B3

验证数据展示

  • Sandwich ELISA standard curve of MP00598-3

    Sandwich ELISA standard curve of MP00598-3

    Sandwich ELISA standard curve of MP00598-3, Rat VEGF Recombinant Matched Antibody Pair - PBS only. 83610-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0941. 83610-2-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

  • Sample test of MP00598-3

    Sample test of MP00598-3

    Rat lung, cut into 1-2 mm pieces, was cultured for 4 days in 25-30 mL of RPMI supplemented with 10% fetal bovine serum and stimulated with 2.5 ng/mL LPS. The cell culture supernatant was removed, assayed for rat VEGF, and measured 3292.9 pg/mL. Rat spleen, cut into 1-2 mm pieces, was cultured for 3-4 days in 25-30 mL of RPMI supplemented with 10% fetal bovine serum and stimulated with 10 μg/mL ConA. The cell culture supernatant was removed, assayed for rat VEGF, and measured 433.1 pg/mL.

  • Sample test of MP00598-3

    Sample test of MP00598-3

    Serum of eight rats was measured. The VEGF 164 concentration of one sample was determined to be 34.3 pg/mL, and the range of VEGF 164 concentration was 0 - 34.3 pg/mL.

  • Cytometric bead array standard curve of MP00598-1

    Cytometric bead array standard curve of MP00598-1

    Cytometric bead array standard curve of MP00598-1, RAT VEGF 164 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83610-2-PBS. Detection antibody: 83610-1-PBS. Standard: Eg0941. Range: 0.313-40 ng/mL.

产品信息

83610-2-PBS targets VEGF 164 as part of a matched antibody pair:

MP00598-1: 83610-2-PBS capture and 83610-1-PBS detection (validated in Cytometric bead array)

MP00598-3: 83610-1-PBS capture and 83610-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity rat
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name vascular endothelial growth factor A
Synonyms Vegfa, VEGF164, Vegf, 240573B3
Calculated Molecular Weight25 kDa
GenBank Accession NumberNM_001287108.1
Gene Symbol Vegfa
Gene ID (NCBI) 83785
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16612-2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C