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验证数据展示

  • IF Staining of mouse brain using CL488-14471

    IF Staining of mouse brain using CL488-14471

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 VGAT antibody (CL488-14471) at dilution of 1:200.

CoraLite® Plus 488-conjugated SLC32A1/VGAT Polyclonal antibody

SLC32A1/VGAT Polyclonal Antibody for IF-P
Cat No. CL488-14471

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

SLC32A1, VGAT, VIAAT, Vesicular GABA transporter, hVIAAT

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 14471-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14471 targets SLC32A1/VGAT in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen SLC32A1/VGAT fusion protein Ag5843 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 32 (GABA vesicular transporter), member 1
Synonyms SLC32A1, VGAT, VIAAT, Vesicular GABA transporter, hVIAAT
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC053582
Gene Symbol VGAT
Gene ID (NCBI) 140679
RRIDAB_3672590
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H598
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC32A1, also known as VGAT (vesicular GABA transporter), functions in the uptake of GABA and glycine into synaptic vesicles. GABA (gamma-aminobutyric acid), is the major inhibitory neurotransmitter in the CNS. VGAT transports GABA and glycine into acidic vesicles and localizes to the synaptic vesicle in glycinergic and GABAergic neurons. And VGAT antibodies are useful markers for presynaptic GABAergic and glycinergic neurons.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SLC32A1/VGAT antibody CL488-14471Download protocol
Standard Protocols
Click here to view our Standard Protocols