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验证数据展示

  • WB analysis of mouse kidney using 16488-1-AP

    WB analysis of mouse kidney using 16488-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16488-1-AP (Villin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse small intestine using 16488-1-AP

    IF Staining of mouse small intestine using 16488-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using Villin antibody (16488-1-AP) at dilution of 1:200 and CoraLite®647-conjugated AffiniPure F(ab, CoraLite® Plus 488 PIGR antibody (CL488-22024, green), CoraLite® Plus 594 ZG16 antibody (CL594-67389, Clone: 1A7B9, red).

  • IF Staining of mouse small intestine using 16488-1-AP

    IF Staining of mouse small intestine using 16488-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Villin antibody (16488-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse kidney using 16488-1-AP

    IP experiment of mouse kidney using 16488-1-AP

    IP result of anti-Villin (IP:16488-1-AP, 4ug; Detection:16488-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

  • IHC staining of mouse small intestine using 16488-1-AP

    IHC staining of mouse small intestine using 16488-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16488-1-AP (Villin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VIL1 antibody included
  • IF Staining of COLO 320 using 16488-1-AP

    IF Staining of COLO 320 using 16488-1-AP

    Immunofluorescent analysis of COLO 320 cells using 16488-1-AP (Villin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 16488-1-AP

    FC experiment of HepG2 using 16488-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Villin (16488-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of mouse small intestine using 16488-1-AP

    IF Staining of mouse small intestine using 16488-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Villin antibody (16488-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

Villin Polyclonal antibody

Villin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Cat No. 16488-1-AP

产品说明书

引用文献(16)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (1)

别名

VIL1, villin 1, VIL, D2S1471

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue, mouse colon tissue, mouse liver tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inmouse small intestine tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue
Positive IF/ICC detected inCOLO 320 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy VIL1 Ready-To-Use IHC Kit. VIL1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16488-1-AP targets Villin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, pig, zebrafish
免疫原 Villin fusion protein Ag9610 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 villin 1
别名 VIL1, villin 1, VIL, D2S1471
计算分子量827aa,93 kDa; 826aa,93 kDa
观测分子量 93 kDa
GenBank蛋白编号BC017303
基因名称 Villin
Gene ID (NCBI) 7429
RRIDAB_2215975
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09327
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Villin 1 (VIL1) is a 95-kd F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma. VIL1 was recently identified as a novel biomarker predictive for postoperative recurrence and poorer prognosis of high serum AFP associated HCC.

实验方案

Product Specific Protocols
WB protocol for Villin antibody 16488-1-APDownload protocol
IHC protocol for Villin antibody 16488-1-APDownload protocol
IF protocol for Villin antibody 16488-1-APDownload protocol
IP protocol for Villin antibody 16488-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Proc Natl Acad Sci U S A

Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface.

Authors - Jacqueline Corry
View Article
mouseIF

Clin Transl Med

NLRP3 inflammasome of renal tubular epithelial cells induces kidney injury in acute hemolytic transfusion reactions.

Authors - Zhixin Liu
View Article
humanWB

mBio

ASB3 expression aggravates inflammatory bowel disease by targeting TRAF6 protein stability and affecting the intestinal microbiota

Authors - Mingyang Cheng
View Article
pigIF

PLoS Pathog

Bile acids promote the caveolae-associated entry of swine acute diarrhea syndrome coronavirus in porcine intestinal enteroids.

Authors - Qi-Yue Yang
View Article
mouseWB

Front Cell Dev Biol

Cathelicidin-WA Protects Against LPS-Induced Gut Damage Through Enhancing Survival and Function of Intestinal Stem Cells.

Authors - Sisi Wang
View Article
mouseIHC

Int J Mol Sci

Nrf2-Knockout Protects from Intestinal Injuries in C57BL/6J Mice Following Abdominal Irradiation with γ Rays.

Authors - Wenyan Yang
View Article