CoraLite® Plus 488-conjugated Villin Polyclonal antibody
Villin Polyclonal Antibody for IF-P
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse small intestine tissue |
Planning an IHC experiment? We recommend our IHCeasy VIL1 Ready-To-Use IHC Kit. VIL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16488-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-16488 targets Villin in IF-P applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | Villin fusion protein Ag9610 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | villin 1 |
| Synonyms | D2S1471, VIL, VIL1, villin 1 |
| Calculated Molecular Weight | 827aa,93 kDa; 826aa,93 kDa |
| Observed Molecular Weight | 93 kDa |
| GenBank Accession Number | BC017303 |
| Gene Symbol | Villin |
| Gene ID (NCBI) | 7429 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P09327 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Villin 1 (VIL1) is a 95-kd F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma. VIL1 was recently identified as a novel biomarker predictive for postoperative recurrence and poorer prognosis of high serum AFP associated HCC.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Villin antibody CL488-16488 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |