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FcZero-rAb™ PE Anti-Human VISTA Rabbit Recombinant Antibody

VISTA FcZero-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

241003C8

Cat no : PE-FcA98109

Print datasheet

Synonyms

C10orf54, SISP1, PP2135, GI24, B7-H5

验证数据展示

  • FC experiment of human peripheral blood leukocyte using PE-FcA98109

    FC experiment of human peripheral blood leukocyte using PE-FcA98109

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul PE Anti-Human VISTA Rabbit Recombinant Antibody (PE-FcA98109, Clone: 241003C8), or 5 ul PE Rabbit IgG Isotype Control Recombinant Antibody (PE-FcA98136, Clone: 240953C9). Cells were not fixed.

经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes
Planning an IHC experiment? We recommend our IHCeasy VISTA Ready-To-Use IHC Kit. VISTA primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-FcA98109 targets VISTA in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chromosome 10 open reading frame 54
Synonyms C10orf54, SISP1, PP2135, GI24, B7-H5
Calculated Molecular Weight 311 aa, 34 kDa
GenBank Accession NumberBC020568
Gene Symbol VISTA
Gene ID (NCBI) 64115
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.

实验方案

Product Specific Protocols
FC protocol for PE VISTA antibody PE-FcA98109Download protocol
Standard Protocols
Click here to view our Standard Protocols