验证数据展示

WB analysis using 11612-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11612-1-AP
WB result of VPRBP antibody (11612-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPRBP transfected HEK-293 cells.
IHC staining of human prostate cancer using 11612-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DCAF1/VPRBP antibody included
IHC staining of human prostate cancer using 11612-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DCAF1/VPRBP antibody included
IP experiment of HeLa using 11612-1-AP
IP result of anti-VPRBP (IP:11612-1-AP, 3ug; Detection:11612-1-AP 1:1000) with HeLa cells lysate 3800ug.
IF Staining of HeLa using 11612-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VPRBP antibody (11612-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HeLa using 11612-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug VPRBP Polyclonal antibody (11612-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

VPRBP Polyclonal antibody

VPRBP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 11612-1-AP

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Publications(42)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Synonyms

KIAA0800, HIV-1 Vpr-binding protein, HIV 1 Vpr binding protein, EC:2.7.11.1, DDB1- and CUL4-associated factor 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®555

Size:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (42)
实验方案

Print datasheet

MSDS

经过测试的应用

Positive WB detected in	DU 145 cells, HeLa cells, mouse testis tissue, PC-3 cells, HEK-293 cells, K-562 cells, HepG2 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human prostate cancer tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy DCAF1/VPRBP Ready-To-Use IHC Kit. DCAF1/VPRBP primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 20 publications below
WB	See 38 publications below
IHC	See 7 publications below
IF	See 6 publications below
IP	See 4 publications below

产品信息

11612-1-AP targets VPRBP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP
Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, canine
Immunogen	VPRBP fusion protein Ag2184 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	Vpr (HIV-1) binding protein
Synonyms	KIAA0800, HIV-1 Vpr-binding protein, HIV 1 Vpr binding protein, EC:2.7.11.1, DDB1- and CUL4-associated factor 1
Calculated Molecular Weight	1506 aa, 169 kDa
Observed Molecular Weight	169 kDa
GenBank Accession Number	BC022792
Gene Symbol	VPRBP
Gene ID (NCBI)	9730
RRID	AB_2216933
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y4B6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507-amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).

实验方案

Product Specific Protocols
WB protocol for VPRBP antibody 11612-1-AP	Download protocol
IHC protocol for VPRBP antibody 11612-1-AP	Download protocol
IF protocol for VPRBP antibody 11612-1-AP	Download protocol
IP protocol for VPRBP antibody 11612-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB,IHC,IF	Science CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins. Authors - Chao Yu KD Validated View Article
mouse, human	WB, IF	Mol Cell CRL4(VprBP) E3 Ligase Promotes Monoubiquitylation and Chromatin Binding of TET Dioxygenases. Authors - Tadashi Nakagawa KD Validated View Article
human	WB	Mol Cell Vpr Targets TET2 for Degradation by CRL4VprBP E3 Ligase to Sustain IL-6 Expression and Enhance HIV-1 Replication. Authors - Lei Lv KD Validated View Article
human	WB	Nat Microbiol Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex. Authors - Leonid Yurkovetskiy View Article
human	WB	Cell Host Microbe Vpr overcomes macrophage-specific restriction of HIV-1 Env expression and virion production. Authors - Michael Mashiba View Article
human	WB, IF	Cell Host Microbe Cyclin L2 Is a Critical HIV Dependency Factor in Macrophages that Controls SAMHD1 Abundance. Authors - George Boateng Kyei KD Validated View Article

验证数据展示

WB analysis using 11612-1-AP

WB analysis of HEK-293 using 11612-1-AP

IHC staining of human prostate cancer using 11612-1-AP

IHC staining of human prostate cancer using 11612-1-AP

IP experiment of HeLa using 11612-1-AP

IF Staining of HeLa using 11612-1-AP

FC experiment of HeLa using 11612-1-AP

VPRBP Polyclonal antibody

VPRBP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

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经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.

CRL4(VprBP) E3 Ligase Promotes Monoubiquitylation and Chromatin Binding of TET Dioxygenases.

Vpr Targets TET2 for Degradation by CRL4VprBP E3 Ligase to Sustain IL-6 Expression and Enhance HIV-1 Replication.

Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex.

Vpr overcomes macrophage-specific restriction of HIV-1 Env expression and virion production.

Cyclin L2 Is a Critical HIV Dependency Factor in Macrophages that Controls SAMHD1 Abundance.