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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated VPRBP Polyclonal antibody

VPRBP Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-11612

Print datasheet

Synonyms

KIAA0800, HIV-1 Vpr-binding protein, HIV 1 Vpr binding protein, EC:2.7.11.1, DDB1- and CUL4-associated factor 1

验证数据展示

  • IF Staining of HeLa using CL647-11612

    IF Staining of HeLa using CL647-11612

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 VPRBP antibody (CL647-11612) at dilution of 1:200.

  • FC experiment of HeLa using CL647-11612

    FC experiment of HeLa using CL647-11612

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human VPRBP (CL647-11612) (red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DCAF1/VPRBP Ready-To-Use IHC Kit. DCAF1/VPRBP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11612-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-11612 targets VPRBP in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen VPRBP fusion protein Ag2184 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Vpr (HIV-1) binding protein
Synonyms KIAA0800, HIV-1 Vpr-binding protein, HIV 1 Vpr binding protein, EC:2.7.11.1, DDB1- and CUL4-associated factor 1
Calculated Molecular Weight 1506 aa, 169 kDa
Observed Molecular Weight 169 kDa
GenBank Accession NumberBC022792
Gene Symbol VPRBP
Gene ID (NCBI) 9730
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4B6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507-amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 VPRBP antibody CL647-11612Download protocol
FC protocol for CL Plus 647 VPRBP antibody CL647-11612Download protocol
Standard Protocols
Click here to view our Standard Protocols