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VPRBP Recombinant antibody, PBS Only

VPRBP Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241028B4

Cat no : 83901-5-PBS

Print datasheet

Synonyms

HIV-1 Vpr-binding protein, HIV 1 Vpr binding protein, EC:2.7.11.1, DDB1- and CUL4-associated factor 1, DCAF1

验证数据展示

  • FC experiment of U-2 OS using 83901-5-RR (same clone as 83901-5-PBS)

    FC experiment of U-2 OS using 83901-5-RR (same clone as 83901-5-PBS)

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug VPRBP Recombinant antibody (83901-5-RR, Clone:241028B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83901-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83901-5-RR (same clone as 83901-5-PBS)

    IF Staining of HeLa using 83901-5-RR (same clone as 83901-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VPRBP antibody (83901-5-RR, Clone: 241028B4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83901-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83901-5-RR

    Affinity and Kinetic Characterization of 83901-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83901-5-RR against Human VPRBP were performed. The affinity constant is 44 pM.

Planning an IHC experiment? We recommend our IHCeasy DCAF1/VPRBP Ready-To-Use IHC Kit. DCAF1/VPRBP primary antibody included.

产品信息

The immunogen of 83901-5-PBS is VPRBP Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen VPRBP fusion protein Ag2184 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Vpr (HIV-1) binding protein
Synonyms HIV-1 Vpr-binding protein, HIV 1 Vpr binding protein, EC:2.7.11.1, DDB1- and CUL4-associated factor 1, DCAF1
Calculated Molecular Weight 1506 aa, 169 kDa
Observed Molecular Weight169 kDa
GenBank Accession NumberBC022792
Gene Symbol VPRBP
Gene ID (NCBI) 9730
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y4B6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507 amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).