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验证数据展示

  • WB analysis using 83901-5-RR

    WB analysis using 83901-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83901-5-RR (VPRBP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of U-2 OS using 83901-5-RR

    FC experiment of U-2 OS using 83901-5-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug VPRBP Recombinant antibody (83901-5-RR, Clone:241028B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IF Staining of HeLa using 83901-5-RR

    IF Staining of HeLa using 83901-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VPRBP antibody (83901-5-RR, Clone: 241028B4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 83901-5-RR

    Affinity and Kinetic Characterization of 83901-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83901-5-RR against Human VPRBP were performed. The affinity constant is 44 pM.

VPRBP Recombinant antibody

VPRBP Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83901-5-RR

产品说明书

CloneNo. 241028B4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

HIV-1 Vpr-binding protein, HIV 1 Vpr binding protein, EC:2.7.11.1, DDB1- and CUL4-associated factor 1, DCAF1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU-2 OS
Planning an IHC experiment? We recommend our IHCeasy DCAF1/VPRBP Ready-To-Use IHC Kit. DCAF1/VPRBP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83901-5-RR targets VPRBP in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 VPRBP fusion protein Ag2184 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Vpr (HIV-1) binding protein
别名 HIV-1 Vpr-binding protein, HIV 1 Vpr binding protein, EC:2.7.11.1, DDB1- and CUL4-associated factor 1, DCAF1
计算分子量 1506 aa, 169 kDa
观测分子量169 kDa
GenBank蛋白编号BC022792
基因名称 VPRBP
Gene ID (NCBI) 9730
RRIDAB_3671483
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9Y4B6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507 amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).

实验方案

Product Specific Protocols
WB protocol for VPRBP antibody 83901-5-RRDownload protocol
IF protocol for VPRBP antibody 83901-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols