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VPS18 Monoclonal antibody, PBS Only

VPS18 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E10G11

Cat no : 67590-1-PBS

Print datasheet

Synonyms

hVPS18, KIAA1475, PEP3, VPS18

验证数据展示

  • WB analysis using 67590-1-Ig (same clone as 67590-1-PBS)

    WB analysis using 67590-1-Ig (same clone as 67590-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67590-1-Ig (VPS18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67590-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67590-1-Ig (same clone as 67590-1-PBS)

    IHC staining of human liver cancer using 67590-1-Ig (same clone as 67590-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67590-1-Ig (VPS18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67590-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67590-1-Ig (same clone as 67590-1-PBS)

    IHC staining of human liver cancer using 67590-1-Ig (same clone as 67590-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67590-1-Ig (VPS18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67590-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 67590-1-Ig (same clone as 67590-1-PBS)

    IF Staining of A549 using 67590-1-Ig (same clone as 67590-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using VPS18 antibody (67590-1-Ig, Clone: 2E10G11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67590-1-PBS in a different storage buffer formulation.

产品信息

67590-1-PBS targets VPS18 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Rat
Immunogen VPS18 fusion protein Ag29954 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name vacuolar protein sorting 18 homolog (S. cerevisiae)
Synonyms hVPS18, KIAA1475, PEP3, VPS18
Calculated Molecular Weight 110 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC001513
Gene Symbol VPS18
Gene ID (NCBI) 57617
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9P253
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Vps18 is a central member of Vps-C complex. Vps18 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.