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VPS18 Monoclonal antibody, PBS Only

VPS18 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2E10G11

Cat No : 67590-1-PBS

Print datasheet

Synonyms

hVPS18, KIAA1475, PEP3, VPS18

验证数据展示

  • WB analysis using 67590-1-Ig (same clone as 67590-1-PBS)

    WB analysis using 67590-1-Ig (same clone as 67590-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67590-1-Ig (VPS18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67590-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67590-1-Ig (same clone as 67590-1-PBS)

    IHC staining of human liver cancer using 67590-1-Ig (same clone as 67590-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67590-1-Ig (VPS18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67590-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67590-1-Ig (same clone as 67590-1-PBS)

    IHC staining of human liver cancer using 67590-1-Ig (same clone as 67590-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67590-1-Ig (VPS18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67590-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 67590-1-Ig (same clone as 67590-1-PBS)

    IF Staining of A549 using 67590-1-Ig (same clone as 67590-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using VPS18 antibody (67590-1-Ig, Clone: 2E10G11 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67590-1-PBS in a different storage buffer formulation.

产品信息

67590-1-PBS targets VPS18 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Rat
Immunogen VPS18 fusion protein Ag29954 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name vacuolar protein sorting 18 homolog (S. cerevisiae)
Synonyms hVPS18, KIAA1475, PEP3, VPS18
Calculated Molecular Weight 110 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC001513
Gene Symbol VPS18
Gene ID (NCBI) 57617
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9P253
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Vps18 is a central member of Vps-C complex. Vps18 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.