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验证数据展示

  • WB analysis using 67590-1-Ig

    WB analysis using 67590-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67590-1-Ig (VPS18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67590-1-Ig

    IHC staining of human liver cancer using 67590-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67590-1-Ig (VPS18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67590-1-Ig

    IHC staining of human liver cancer using 67590-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67590-1-Ig (VPS18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 67590-1-Ig

    IF Staining of A549 using 67590-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using VPS18 antibody (67590-1-Ig, Clone: 2E10G11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

VPS18 Monoclonal antibody

VPS18 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67590-1-Ig

产品说明书

CloneNo. 2E10G11

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Rat

应用

WB, IHC, IF/ICC, ELISA

别名

hVPS18, KIAA1475, PEP3, VPS18

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67590-1-Ig targets VPS18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Rat
免疫原 VPS18 fusion protein Ag29954 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 vacuolar protein sorting 18 homolog (S. cerevisiae)
别名 hVPS18, KIAA1475, PEP3, VPS18
计算分子量 110 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC001513
基因名称 VPS18
Gene ID (NCBI) 57617
RRIDAB_2882798
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9P253
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Vps18 is a central member of Vps-C complex. Vps18 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

实验方案

Product Specific Protocols
WB protocol for VPS18 antibody 67590-1-IgDownload protocol
IHC protocol for VPS18 antibody 67590-1-IgDownload protocol
IF protocol for VPS18 antibody 67590-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols