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CoraLite® Plus 488-conjugated VPS18 Monoclonal antibody

VPS18 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E10G11

Cat no : CL488-67590

Print datasheet

Synonyms

hVPS18, KIAA1475, PEP3, VPS18

验证数据展示

  • IF Staining of A549 using CL488-67590

    IF Staining of A549 using CL488-67590

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 VPS18 antibody (CL488-67590, Clone: 2E10G11 ) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 67590-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67590 targets VPS18 in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen VPS18 fusion protein Ag29954 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name vacuolar protein sorting 18 homolog (S. cerevisiae)
Synonyms hVPS18, KIAA1475, PEP3, VPS18
Calculated Molecular Weight 110 kDa
GenBank Accession NumberBC001513
Gene Symbol VPS18
Gene ID (NCBI) 57617
RRIDAB_2919503
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9P253
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Vps18 is a central member of Vps-C complex. Vps18 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 VPS18 antibody CL488-67590Download protocol
Standard Protocols
Click here to view our Standard Protocols