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VPS25 Monoclonal antibody, PBS Only

VPS25 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rabbit

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B4C3

Cat no : 68197-1-PBS

Print datasheet

Synonyms

DERP9, EAP20, ESCRT II complex subunit VPS25, FAP20, hVps25, VPS25

验证数据展示

  • WB analysis using 68197-1-Ig (same clone as 68197-1-PBS)

    WB analysis using 68197-1-Ig (same clone as 68197-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68197-1-Ig (VPS25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68197-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68197-1-Ig (same clone as 68197-1-PBS)

    IF Staining of HepG2 using 68197-1-Ig (same clone as 68197-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VPS25 antibody (68197-1-Ig, Clone: 3B4C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68197-1-PBS in a different storage buffer formulation.

  • WB analysis of rabbit kidney using 68197-1-Ig (same clone as 68197-1-PBS)

    WB analysis of rabbit kidney using 68197-1-Ig (same clone as 68197-1-PBS)

    rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68197-1-Ig (VPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68197-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy VPS25 Ready-To-Use IHC Kit. VPS25 primary antibody included.

产品信息

68197-1-PBS targets VPS25 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Rabbit samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Rabbit
Immunogen VPS25 fusion protein Ag8243 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vacuolar protein sorting 25 homolog (S. cerevisiae)
Synonyms DERP9, EAP20, ESCRT II complex subunit VPS25, FAP20, hVps25, VPS25
Calculated Molecular Weight 176 aa, 21 kDa
Observed Molecular Weight19 kDa
GenBank Accession NumberBC006282
Gene Symbol VPS25
Gene ID (NCBI) 84313
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BRG1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VPS25 is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT-II complex, composed of VPS25, VPS36, and SNF8, functions in sorting of ubiquitinated membrane proteins during endocytosis. ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is highly conserved. Human VPS25 gene maps to chromosome 17q21.31 and is expressed in a wide range of tissues (PMID: 16889659).