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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68197-1-Ig (same clone as 68197-1-PBS)

    WB analysis using 68197-1-Ig (same clone as 68197-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68197-1-Ig (VPS25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68197-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68197-1-Ig (same clone as 68197-1-PBS)

    IF Staining of HepG2 using 68197-1-Ig (same clone as 68197-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VPS25 antibody (68197-1-Ig, Clone: 3B4C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68197-1-PBS in a different storage buffer formulation.

  • WB analysis of rabbit kidney using 68197-1-Ig (same clone as 68197-1-PBS)

    WB analysis of rabbit kidney using 68197-1-Ig (same clone as 68197-1-PBS)

    rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68197-1-Ig (VPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68197-1-PBS in a different storage buffer formulation.

VPS25 Monoclonal antibody, PBS Only

VPS25 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68197-1-PBS

产品说明书

CloneNo. 3B4C3

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rabbit

应用

WB, IF/ICC, Indirect ELISA

别名

DERP9, EAP20, ESCRT II complex subunit VPS25, FAP20, hVps25, VPS25

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy VPS25 Ready-To-Use IHC Kit. VPS25 primary antibody included.

产品信息

68197-1-PBS targets VPS25 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Rabbit samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Rabbit
免疫原 VPS25 fusion protein Ag8243 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 vacuolar protein sorting 25 homolog (S. cerevisiae)
别名 DERP9, EAP20, ESCRT II complex subunit VPS25, FAP20, hVps25, VPS25
计算分子量 176 aa, 21 kDa
观测分子量19 kDa
GenBank蛋白编号BC006282
基因名称 VPS25
Gene ID (NCBI) 84313
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BRG1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VPS25 is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT-II complex, composed of VPS25, VPS36, and SNF8, functions in sorting of ubiquitinated membrane proteins during endocytosis. ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is highly conserved. Human VPS25 gene maps to chromosome 17q21.31 and is expressed in a wide range of tissues (PMID: 16889659).