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VPS25 Monoclonal antibody

VPS25 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3B4C3

Cat no : 68197-1-Ig

Print datasheet

Synonyms

DERP9, EAP20, ESCRT II complex subunit VPS25, FAP20, hVps25, VPS25

验证数据展示

  • WB analysis using 68197-1-Ig

    WB analysis using 68197-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68197-1-Ig (VPS25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68197-1-Ig

    IF Staining of HepG2 using 68197-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VPS25 antibody (68197-1-Ig, Clone: 3B4C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • WB analysis of rabbit kidney using 68197-1-Ig

    WB analysis of rabbit kidney using 68197-1-Ig

    rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68197-1-Ig (VPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, rabbit kidney tissue, HepG2 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy VPS25 Ready-To-Use IHC Kit. VPS25 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68197-1-Ig targets VPS25 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Rabbit samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, Rabbit
Immunogen VPS25 fusion protein Ag8243 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vacuolar protein sorting 25 homolog (S. cerevisiae)
Synonyms DERP9, EAP20, ESCRT II complex subunit VPS25, FAP20, hVps25, VPS25
Calculated Molecular Weight 176 aa, 21 kDa
Observed Molecular Weight19 kDa
GenBank Accession NumberBC006282
Gene Symbol VPS25
Gene ID (NCBI) 84313
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BRG1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS25 is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT-II complex, composed of VPS25, VPS36, and SNF8, functions in sorting of ubiquitinated membrane proteins during endocytosis. ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is highly conserved. Human VPS25 gene maps to chromosome 17q21.31 and is expressed in a wide range of tissues (PMID: 16889659).

实验方案

Product Specific Protocols
WB protocol for VPS25 antibody 68197-1-IgDownload protocol
IF protocol for VPS25 antibody 68197-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols