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验证数据展示

  • IF Staining of HepG2 using CL488-68197

    IF Staining of HepG2 using CL488-68197

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 VPS25 antibody (CL488-68197, Clone: 3B4C3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated VPS25 Monoclonal antibody

VPS25 Monoclonal Antibody for IF/ICC
Cat No. CL488-68197

产品说明书

CloneNo. 3B4C3

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rabbit

应用

IF/ICC

别名

DERP9, EAP20, ESCRT II complex subunit VPS25, FAP20, hVps25, VPS25

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy VPS25 Ready-To-Use IHC Kit. VPS25 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68197-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68197 targets VPS25 in IF/ICC applications and shows reactivity with Human, Rabbit samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Rabbit
免疫原 VPS25 fusion protein Ag8243 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 vacuolar protein sorting 25 homolog (S. cerevisiae)
别名 DERP9, EAP20, ESCRT II complex subunit VPS25, FAP20, hVps25, VPS25
计算分子量 176 aa, 21 kDa
观测分子量19 kDa
GenBank蛋白编号BC006282
基因名称 VPS25
Gene ID (NCBI) 84313
RRIDAB_3084438
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BRG1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS25 is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT-II complex, composed of VPS25, VPS36, and SNF8, functions in sorting of ubiquitinated membrane proteins during endocytosis. ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is highly conserved. Human VPS25 gene maps to chromosome 17q21.31 and is expressed in a wide range of tissues (PMID: 16889659).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 VPS25 antibody CL488-68197Download protocol
Standard Protocols
Click here to view our Standard Protocols