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验证数据展示

  • IF Staining of HepG2 using CL488-68197

    IF Staining of HepG2 using CL488-68197

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 VPS25 antibody (CL488-68197, Clone: 3B4C3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated VPS25 Monoclonal antibody

VPS25 Monoclonal Antibody for IF/ICC
Cat No. CL488-68197

Print datasheet

CloneNo. 3B4C3

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rabbit

Applications

IF/ICC

Synonyms

DERP9, EAP20, ESCRT II complex subunit VPS25, FAP20, hVps25, VPS25

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy VPS25 Ready-To-Use IHC Kit. VPS25 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68197-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68197 targets VPS25 in IF/ICC applications and shows reactivity with Human, Rabbit samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Rabbit
Immunogen VPS25 fusion protein Ag8243 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vacuolar protein sorting 25 homolog (S. cerevisiae)
Synonyms DERP9, EAP20, ESCRT II complex subunit VPS25, FAP20, hVps25, VPS25
Calculated Molecular Weight 176 aa, 21 kDa
Observed Molecular Weight19 kDa
GenBank Accession NumberBC006282
Gene Symbol VPS25
Gene ID (NCBI) 84313
RRIDAB_3084438
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BRG1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS25 is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT-II complex, composed of VPS25, VPS36, and SNF8, functions in sorting of ubiquitinated membrane proteins during endocytosis. ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is highly conserved. Human VPS25 gene maps to chromosome 17q21.31 and is expressed in a wide range of tissues (PMID: 16889659).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 VPS25 antibody CL488-68197Download protocol
Standard Protocols
Click here to view our Standard Protocols