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  • Featured Product
  • KD/KO Validated

VPS28 Polyclonal antibody, PBS Only

VPS28 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15478-1-PBS

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Synonyms

验证数据展示

  • WB analysis of mouse brain using 15478-1-AP (same clone as 15478-1-PBS)

    WB analysis of mouse brain using 15478-1-AP (same clone as 15478-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15478-1-AP (VPS28 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 15478-1-AP (same clone as 15478-1-PBS)

    IP experiment of mouse brain using 15478-1-AP (same clone as 15478-1-PBS)

    IP result of anti-VPS28 (IP:15478-1-AP, 4ug; Detection:15478-1-AP 1:500) with mouse brain tissue lysate 4400ug. This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 15478-1-AP (same clone as 15478-1-PBS)

    IF Staining of HepG2 using 15478-1-AP (same clone as 15478-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 15478-1-AP (VPS28 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

产品信息

15478-1-PBS targets VPS28 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen VPS28 fusion protein Ag7784 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name vacuolar protein sorting 28 homolog (S. cerevisiae)
Synonyms
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC006485
Gene Symbol VPS28
Gene ID (NCBI) 51160
RRIDAB_2878144
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UK41
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described.