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VPS45 Polyclonal antibody, PBS Only

VPS45 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12006-1-PBS

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Synonyms

验证数据展示

  • IHC staining of mouse brain using 12006-1-AP (same clone as 12006-1-PBS)

    IHC staining of mouse brain using 12006-1-AP (same clone as 12006-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12006-1-AP (VPS45 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 12006-1-AP (same clone as 12006-1-PBS)

    IHC staining of mouse brain using 12006-1-AP (same clone as 12006-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12006-1-AP (VPS45 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12006-1-AP (same clone as 12006-1-PBS)

    IF Staining of HeLa using 12006-1-AP (same clone as 12006-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12006-1-AP (VPS45 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

产品信息

12006-1-PBS targets VPS45 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen VPS45 fusion protein Ag2658 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name vacuolar protein sorting 45 homolog (S. cerevisiae)
Synonyms
Calculated Molecular Weight 570 aa, 65 kDa
GenBank Accession NumberBC012932
Gene Symbol VPS45
Gene ID (NCBI) 11311
RRIDAB_2215369
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRW7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)