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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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验证数据展示

  • WB analysis of HepG2 using 11662-2-AP (same clone as 11662-2-PBS)

    WB analysis of HepG2 using 11662-2-AP (same clone as 11662-2-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11662-2-AP (VPS52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11662-2-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 11662-2-AP (same clone as 11662-2-PBS)

    IHC staining of human gliomas using 11662-2-AP (same clone as 11662-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11662-2-AP (VPS52 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11662-2-PBS in a different storage buffer formulation.

  • IP experiment of mouse skin using 11662-2-AP (same clone as 11662-2-PBS)

    IP experiment of mouse skin using 11662-2-AP (same clone as 11662-2-PBS)

    IP result of anti-VPS52 (IP:11662-2-AP, 3ug; Detection:11662-2-AP 1:1000) with mouse skin tissue lysate 3200ug. This data was developed using the same antibody clone with 11662-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 11662-2-AP (same clone as 11662-2-PBS)

    IF Staining of HepG2 using 11662-2-AP (same clone as 11662-2-PBS)

    Immunofluorescent analysis of HepG2 cells using 11662-2-AP (VPS52 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11662-2-PBS in a different storage buffer formulation.

VPS52 Polyclonal antibody, PBS Only

VPS52 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11662-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

11662-2-PBS targets VPS52 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 VPS52 fusion protein Ag2180 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vacuolar protein sorting 52 homolog (S. cerevisiae)
别名
计算分子量 723 aa, 82 kDa
观测分子量 82 kDa
GenBank蛋白编号BC032108
基因名称 VPS52
Gene ID (NCBI) 6293
RRIDAB_2241614
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N1B4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VPS52 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins. VPS52 may play a role in the development of a multicellular organism through cell-cell interactions (PMID: 23142660).