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VPS52 Polyclonal antibody, PBS Only

VPS52 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11662-2-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis of HepG2 using 11662-2-AP (same clone as 11662-2-PBS)

    WB analysis of HepG2 using 11662-2-AP (same clone as 11662-2-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11662-2-AP (VPS52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11662-2-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 11662-2-AP (same clone as 11662-2-PBS)

    IHC staining of human gliomas using 11662-2-AP (same clone as 11662-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11662-2-AP (VPS52 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11662-2-PBS in a different storage buffer formulation.

  • IP experiment of mouse skin using 11662-2-AP (same clone as 11662-2-PBS)

    IP experiment of mouse skin using 11662-2-AP (same clone as 11662-2-PBS)

    IP result of anti-VPS52 (IP:11662-2-AP, 3ug; Detection:11662-2-AP 1:1000) with mouse skin tissue lysate 3200ug. This data was developed using the same antibody clone with 11662-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 11662-2-AP (same clone as 11662-2-PBS)

    IF Staining of HepG2 using 11662-2-AP (same clone as 11662-2-PBS)

    Immunofluorescent analysis of HepG2 cells using 11662-2-AP (VPS52 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11662-2-PBS in a different storage buffer formulation.

产品信息

11662-2-PBS targets VPS52 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen VPS52 fusion protein Ag2180 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name vacuolar protein sorting 52 homolog (S. cerevisiae)
Synonyms
Calculated Molecular Weight 723 aa, 82 kDa
Observed Molecular Weight 82 kDa
GenBank Accession NumberBC032108
Gene Symbol VPS52
Gene ID (NCBI) 6293
RRIDAB_2241614
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N1B4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VPS52 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins. VPS52 may play a role in the development of a multicellular organism through cell-cell interactions (PMID: 23142660).