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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67610-1-Ig (same clone as 67610-1-PBS)

    WB analysis using 67610-1-Ig (same clone as 67610-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67610-1-Ig (VPS53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 67610-1-Ig (same clone as 67610-1-PBS)

    IHC staining of human liver using 67610-1-Ig (same clone as 67610-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67610-1-Ig (VPS53 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VPS53 antibody included
  • IHC staining of human liver using 67610-1-Ig (same clone as 67610-1-PBS)

    IHC staining of human liver using 67610-1-Ig (same clone as 67610-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67610-1-Ig (VPS53 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VPS53 antibody included
  • IF Staining of HEK-293 using 67610-1-Ig (same clone as 67610-1-PBS)

    IF Staining of HEK-293 using 67610-1-Ig (same clone as 67610-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 67610-1-Ig (VPS53 antibody), at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

VPS53 Monoclonal antibody, PBS Only

VPS53 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67610-1-PBS

产品说明书

CloneNo. 2B12C3

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

FLJ10979, HCCS1, hVps53L, pp13624, VPS53

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy VPS53 Ready-To-Use IHC Kit. VPS53 primary antibody included.

产品信息

67610-1-PBS targets VPS53 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 VPS53 fusion protein Ag29875 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 vacuolar protein sorting 53 homolog (S. cerevisiae)
别名 FLJ10979, HCCS1, hVps53L, pp13624, VPS53
计算分子量 832 aa, 94 kDa
观测分子量 94 kDa
GenBank蛋白编号BC029560
基因名称 VPS53
Gene ID (NCBI) 55275
RRIDAB_2882816
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ5VIR6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VPS53 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes to the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins.