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VPS53 Monoclonal antibody

VPS53 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B12C3

Cat no : 67610-1-Ig

Print datasheet

Synonyms

FLJ10979, HCCS1, hVps53L, pp13624, VPS53

验证数据展示

  • WB analysis using 67610-1-Ig

    WB analysis using 67610-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67610-1-Ig (VPS53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • IHC staining of human liver using 67610-1-Ig

    IHC staining of human liver using 67610-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67610-1-Ig (VPS53 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VPS53 antibody included
  • IHC staining of human liver using 67610-1-Ig

    IHC staining of human liver using 67610-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67610-1-Ig (VPS53 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VPS53 antibody included
  • IF Staining of HEK-293 using 67610-1-Ig

    IF Staining of HEK-293 using 67610-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 67610-1-Ig (VPS53 antibody), at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inU2OS cells, HeLa cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, LNCaP cells, 4T1 cells
Positive IHC detected inhuman liver tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy VPS53 Ready-To-Use IHC Kit. VPS53 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®555 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67610-1-Ig targets VPS53 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen VPS53 fusion protein Ag29875 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vacuolar protein sorting 53 homolog (S. cerevisiae)
Synonyms FLJ10979, HCCS1, hVps53L, pp13624, VPS53
Calculated Molecular Weight 832 aa, 94 kDa
Observed Molecular Weight 94 kDa
GenBank Accession NumberBC029560
Gene Symbol VPS53
Gene ID (NCBI) 55275
RRIDAB_2882816
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ5VIR6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS53 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes to the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins.

实验方案

Product Specific Protocols
WB protocol for VPS53 antibody 67610-1-IgDownload protocol
IHC protocol for VPS53 antibody 67610-1-IgDownload protocol
IF protocol for VPS53 antibody 67610-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols