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CoraLite®555-conjugated VPS53 Monoclonal antibody

VPS53 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2B12C3

Cat no : CL555-67610

Print datasheet

Synonyms

FLJ10979, HCCS1, hVps53L, pp13624, VPS53

验证数据展示

  • IF Staining of HEK-293 using CL555-67610

    IF Staining of HEK-293 using CL555-67610

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®555 VPS53 antibody (CL555-67610, Clone: 2B12C3 ) at dilution of 1:400.

经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy VPS53 Ready-To-Use IHC Kit. VPS53 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67610-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-67610 targets VPS53 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen VPS53 fusion protein Ag29875 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vacuolar protein sorting 53 homolog (S. cerevisiae)
Synonyms FLJ10979, HCCS1, hVps53L, pp13624, VPS53
Calculated Molecular Weight 832 aa, 94 kDa
Observed Molecular Weight 94 kDa
GenBank Accession NumberBC029560
Gene Symbol VPS53
Gene ID (NCBI) 55275
RRIDAB_2934684
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ5VIR6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS53 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes to the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins.

实验方案

Product Specific Protocols
IF protocol for CL555 VPS53 antibody CL555-67610Download protocol
Standard Protocols
Click here to view our Standard Protocols