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VWA1 Polyclonal antibody

VWA1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14322-1-AP

Print datasheet

Synonyms

VWA 1, VWA1, WARP

验证数据展示

  • WB analysis of T-47D using 14322-1-AP

    WB analysis of T-47D using 14322-1-AP

    T-47D cells were subjected to SDS PAGE followed by western blot with 14322-1-AP (VWA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 14322-1-AP

    IHC staining of human pancreas cancer using 14322-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14322-1-AP (VWA1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 14322-1-AP

    IHC staining of human pancreas cancer using 14322-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14322-1-AP (VWA1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inT-47D cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14322-1-AP targets VWA1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen VWA1 fusion protein Ag5565 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name von Willebrand factor A domain containing 1
Synonyms VWA 1, VWA1, WARP
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC059409
Gene Symbol VWA1
Gene ID (NCBI) 64856
RRIDAB_2241694
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6PCB0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VWA1 (also known as WARP) is a member of the von Willebrand factor A-domain (VA-domain) superfamily of extracellular matrix proteins which may play a role in cartilage structure and function (PMID: 12062410). VWA1 is a multimeric component of the chondrocyte pericellular matrix in articular cartilage and intervertebral disc, where it interacts with the basement membrane heparan sulfate proteoglycan perlecan (PMID: 18314316). VWA1 may have a role in maintaining the blood-brain barrier.

实验方案

Product Specific Protocols
WB protocol for VWA1 antibody 14322-1-APDownload protocol
IHC protocol for VWA1 antibody 14322-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Theranostics

Survey of the translation shifts in hepatocellular carcinoma with ribosome profiling.

Authors - Qin Zou
View Article