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验证数据展示

  • WB analysis of T-47D using 14322-1-AP

    WB analysis of T-47D using 14322-1-AP

    T-47D cells were subjected to SDS PAGE followed by western blot with 14322-1-AP (VWA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 14322-1-AP

    IHC staining of human pancreas cancer using 14322-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14322-1-AP (VWA1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 14322-1-AP

    IHC staining of human pancreas cancer using 14322-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14322-1-AP (VWA1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

VWA1 Polyclonal antibody

VWA1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14322-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

VWA 1, VWA1, WARP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inT-47D cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14322-1-AP targets VWA1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 VWA1 fusion protein Ag5565 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 von Willebrand factor A domain containing 1
别名 VWA 1, VWA1, WARP
计算分子量 47 kDa
观测分子量 48 kDa
GenBank蛋白编号BC059409
基因名称 VWA1
Gene ID (NCBI) 64856
RRIDAB_2241694
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6PCB0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VWA1 (also known as WARP) is a member of the von Willebrand factor A-domain (VA-domain) superfamily of extracellular matrix proteins which may play a role in cartilage structure and function (PMID: 12062410). VWA1 is a multimeric component of the chondrocyte pericellular matrix in articular cartilage and intervertebral disc, where it interacts with the basement membrane heparan sulfate proteoglycan perlecan (PMID: 18314316). VWA1 may have a role in maintaining the blood-brain barrier.

实验方案

Product Specific Protocols
WB protocol for VWA1 antibody 14322-1-APDownload protocol
IHC protocol for VWA1 antibody 14322-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Theranostics

Survey of the translation shifts in hepatocellular carcinoma with ribosome profiling.

Authors - Qin Zou
View Article