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CoraLite® Plus 488-conjugated ValRS Monoclonal antibody

ValRS Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A2C3

Cat no : CL488-67935

Print datasheet

Synonyms

Valyl-tRNA synthetase, VARS, valyl tRNA synthetase, Valine--tRNA ligase, Valine tRNA ligase

验证数据展示

  • IF Staining of HeLa using CL488-67935

    IF Staining of HeLa using CL488-67935

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 ValRS antibody (CL488-67935, Clone: 1A2C3 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-67935

    FC experiment of HeLa using CL488-67935

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ValRS (CL488-67935, Clone:1A2C3) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67935-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67935 targets ValRS in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen ValRS fusion protein Ag9312 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name valyl-tRNA synthetase
Synonyms Valyl-tRNA synthetase, VARS, valyl tRNA synthetase, Valine--tRNA ligase, Valine tRNA ligase
Calculated Molecular Weight 1264 aa, 140 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC012808
Gene Symbol ValRS
Gene ID (NCBI) 7407
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26640
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ValRS antibody CL488-67935Download protocol
FC protocol for CL Plus 488 ValRS antibody CL488-67935Download protocol
Standard Protocols
Click here to view our Standard Protocols