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验证数据展示

  • IHC staining of human liver cancer using 67620-1-Ig

    IHC staining of human liver cancer using 67620-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 67620-1-Ig

    IF Staining of mouse brain using 67620-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using WASF3 antibody (67620-1-Ig, Clone: 3B7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

WASF3 Monoclonal antibody

WASF3 Monoclonal Antibody for IHC, IF-P, ELISA
Cat No. 67620-1-Ig

产品说明书

CloneNo. 3B7D10

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Pig, Rabbit

应用

IHC, IF-P, ELISA

别名

Brush 1, KIAA0900, Protein WAVE 3, SCAR3, WAS protein family, member 3, WASF3, WASP family protein member 3, WAVE3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue, human liver cancer tissue
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67620-1-Ig targets WASF3 in IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig, Rabbit
免疫原 WASF3 fusion protein Ag29991 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 WAS protein family, member 3
别名 Brush 1, KIAA0900, Protein WAVE 3, SCAR3, WAS protein family, member 3, WASF3, WASP family protein member 3, WAVE3
计算分子量 55 kDa
观测分子量 50-55 and 66-70 kDa
GenBank蛋白编号BC050283
基因名称 WASF3
Gene ID (NCBI) 10810
RRIDAB_2882822
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UPY6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WASF3, also named WAVE3 or SCAR3, belongs to the SCAR/WAVE family. It plays a role in the regulation of cell morphology and cytoskeletal organization. WASF3 is associated with movement, invasion, and metastasis in different cancers such as breast cancer, gastric cancer, and prostate cancer. It has been shown that WASF3 levels are elevated in advanced-stage cancers compared to lower-grade tumors and normal tissue. The 67620-1-Ig antibody recognizes 50-55 kDa and 66-70 kDa proteins similar to the papers.(PMID: 21544801, 15826941, 31038356, 27432794, 31542393)

实验方案

Product Specific Protocols
WB protocol for WASF3 antibody 67620-1-IgDownload protocol
IHC protocol for WASF3 antibody 67620-1-IgDownload protocol
IF protocol for WASF3 antibody 67620-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols