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验证数据展示

  • IF Staining of mouse brain using CL647-67620

    IF Staining of mouse brain using CL647-67620

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 WASF3 antibody (CL647-67620, Clone: 3B7D10 ) at dilution of 1:200.

CoraLite® Plus 647-conjugated WASF3 Monoclonal antibody

WASF3 Monoclonal Antibody for IF-P
Cat No. CL647-67620

产品说明书

CloneNo. 3B7D10

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Pig, Rabbit

应用

IF-P

别名

Brush 1, KIAA0900, Protein WAVE 3, SCAR3, WAS protein family, member 3, WASF3, WASP family protein member 3, WAVE3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 67620-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67620 targets WASF3 in IF-P applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

经测试应用 IF-P Application Description
经测试反应性 Human, Mouse, Rat, Pig, Rabbit
免疫原 WASF3 fusion protein Ag29991 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 WAS protein family, member 3
别名 Brush 1, KIAA0900, Protein WAVE 3, SCAR3, WAS protein family, member 3, WASF3, WASP family protein member 3, WAVE3
计算分子量 55 kDa
观测分子量 50-55 and 66-70 kDa
GenBank蛋白编号BC050283
基因名称 WASF3
Gene ID (NCBI) 10810
RRIDAB_3084825
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UPY6
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WASF3, also named as WAVE3 or SCAR3, belongs to the SCAR/WAVE family. It plays a role in the regulation of cell morphology and cytoskeletal organization. WASF3 is associated with the movement, invasion and metastasis in different cancers such as breast cancer, gastric cancer and prostate cancer. It has been shown that WASF3 levels are elevated in advanced stage cancers compared to lower grade tumors and normal tissue. 67620-1-Ig antibody recognizes 50-55 kDa and 66-70 kDa protein similar to the papers.(PMID: 21544801, 15826941, 31038356, 27432794, 31542393)

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 WASF3 antibody CL647-67620Download protocol
Standard Protocols
Click here to view our Standard Protocols