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WDR32 Polyclonal antibody

WDR32 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20483-1-AP

Print datasheet

Synonyms

DCAF10, WD repeat domain 32, WDR32

验证数据展示

  • WB analysis of HeLa using 20483-1-AP

    WB analysis of HeLa using 20483-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20483-1-AP (WDR32 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 20483-1-AP

    IP experiment of HepG2 using 20483-1-AP

    IP result of anti-WDR32 (IP:20483-1-AP, 4ug; Detection:20483-1-AP 1:300) with HepG2 cells lysate 6000ug.

  • IHC staining of human testis using 20483-1-AP

    IHC staining of human testis using 20483-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20483-1-AP (WDR32 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 20483-1-AP

    IHC staining of human testis using 20483-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20483-1-AP (WDR32 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 20483-1-AP

    IF Staining of HepG2 using 20483-1-AP

    Immunofluorescent analysis of HepG2 cells using 20483-1-AP (WDR32 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20483-1-AP is WDR32 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen WDR32 fusion protein Ag14360 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WD repeat domain 32
Synonyms DCAF10, WD repeat domain 32, WDR32
Calculated Molecular Weight 559 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC003520
Gene Symbol WDR32
Gene ID (NCBI) 79269
RRIDAB_10695183
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5QP82
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WDR32, containing seven WD repeats, is also termed DDB1- and CUL4-associated factor 10 (DCAF10), which may function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex (PMID: 16949367). WDR32 is able to interact with DDB1 (PMID: 16949367). WDR32 is supposed to be implicated in process of ubiquitin-dependent protein degradation system. Two isoforms are described and produced by alternative splicing. Proteintech's WDR32 antibody 20483-1-AP majorly detects WDR32 isoform1 with a theoretical MW of 61 kDa.

实验方案

Product Specific Protocols
WB protocol for WDR32 antibody 20483-1-APDownload protocol
IHC protocol for WDR32 antibody 20483-1-APDownload protocol
IF protocol for WDR32 antibody 20483-1-APDownload protocol
IP protocol for WDR32 antibody 20483-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols