WDR5 Polyclonal antibody
WDR5 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, K-562 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy WDR5 Ready-To-Use IHC Kit. WDR5 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15544-1-AP targets WDR5 in WB, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF, CoIP, ChIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | WDR5 fusion protein Ag7900 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | WD repeat domain 5 |
| Synonyms | BIG 3, BIG3, BMP2 induced 3 kb gene protein, SWD3, WD repeat containing protein 5, WD repeat domain 5, WDR5 |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC001635 |
| Gene Symbol | WDR5 |
| Gene ID (NCBI) | 11091 |
| RRID | AB_2257220 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P61964 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The WDR5 antibody targets the WD repeat protein domain 5 (WDR5), a member of the WD40 repeat protein family in humans. The WDR5 protein is a core member of the mammalian trithorax complex and, like the rest of the WD40 repeat protein family, is primarily involved in diverse cellular processes. WDR5 is identified as a methyl Lys-4 H3-specific-binding protein, however it is known to interact with the Mixed Lineage Leukemia (MLL) protein at the same site for histone H3 tri-methylation(PMID:18840606). Recently, the WDR5 protein has been shown to regulate embryonic stem cell self-renewal, and an increased expression of WDR5 has been found to efficiently induce the formation of pluripotent stem cells (PMID:21477851). WDR5 is also shown to accelerate osteoblast and chondrocyte differentiation(PMID:16730692 ).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR5 antibody 15544-1-AP | Download protocol |
| IHC protocol for WDR5 antibody 15544-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics | ||
Sci Adv A MYC inhibitor selectively alters the MYC and MAX cistromes and modulates the epigenomic landscape to regulate target gene expression. | ||
Proc Natl Acad Sci U S A Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. | ||
Pharmacol Res HPV E7-drived ALKBH5 promotes cervical cancer progression by modulating m6A modification of PAK5 | ||
Nat Aging Transcriptional and epigenetic dysregulation impairs generation of proliferative neural stem and progenitor cells during brain aging | ||
Mol Med Glucagon-like peptide-1 receptor agonist exendin 4 ameliorates diabetes-associated vascular calcification by regulating mitophagy through the AMPK signaling pathway |