IHCeasy WDR5 Ready-To-Use IHC Kit
WDR5 Ready-to-use reagent kit for IHC.
Reactivity
Human, Mouse, Rat
Sample Type
FFPE tissue
Cat No : KHC1643
Synonyms
BIG 3, BIG3, BMP2 induced 3 kb gene protein, SWD3, WD repeat containing protein 5, WD repeat domain 5, WDR5
Validation Data Gallery
Product Information
KHC1643 is a ready-to-use IHC kit for staining of WDR5. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy WDR5 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
The WDR5 antibody targets the WD repeat protein domain 5 (WDR5), a member of the WD40 repeat protein family in humans. The WDR5 protein is a core member of the mammalian trithorax complex and, like the rest of the WD40 repeat protein family, is primarily involved in diverse cellular processes. WDR5 is identified as a methyl Lys-4 H3-specific-binding protein, however it is known to interact with the Mixed Lineage Leukemia (MLL) protein at the same site for histone H3 tri-methylation. Recently, the WDR5 protein has been shown to regulate embryonic stem cell self-renewal, and an increased expression of WDR5 has been found to efficiently induce the formation of pluripotent stem cells. WDR5 is also shown to accelerate osteoblast and chondrocyte differentiation.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | BIG 3, BIG3, BMP2 induced 3 kb gene protein, SWD3, WD repeat containing protein 5, WD repeat domain 5, WDR5 |
