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CoraLite® Plus 488-conjugated WDR55 Polyclonal antibody

WDR55 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-20786

Print datasheet

Synonyms

WD repeat-containing protein 55, WD repeat domain 55

验证数据展示

  • IF Staining of HepG2 using CL488-20786

    IF Staining of HepG2 using CL488-20786

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 WDR55 antibody (CL488-20786) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-20786

    FC experiment of HepG2 using CL488-20786

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated WDR55 Polyclonal antibody (CL488-20786)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 20786-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-20786 targets WDR55 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen WDR55 fusion protein Ag14241 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WD repeat domain 55
Synonyms WD repeat-containing protein 55, WD repeat domain 55
Calculated Molecular Weight 383 aa, 42 kDa
Observed Molecular Weight 42-50 kDa
GenBank Accession NumberBC002482
Gene Symbol WDR55
Gene ID (NCBI) 54853
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H6Y2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WD repeat domain 55 (WDR55) also named as, FLJ20195, is a 383 amino acid protein, which contains seven WD repeats and belongs to the WD repeat WDR55 family. WDR55 localizes in the nucleus and cytoplasm. WDR55 acts as a modulator of rRNA synthesis and play a central role in organogenesis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 WDR55 antibody CL488-20786Download protocol
FC protocol for CL Plus 488 WDR55 antibody CL488-20786Download protocol
Standard Protocols
Click here to view our Standard Protocols