验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse liver tissue, HepG2 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse testis tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IP | See 1 publications below |
产品信息
22536-1-AP targets WDR61 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | WDR61 fusion protein Ag18443 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | WD repeat domain 61 |
| 别名 | WD repeat domain 61, Superkiller complex protein 8, N-terminally processed, Superkiller complex protein 8, SKIC8, Ski8 |
| 计算分子量 | 305 aa, 34 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC010080 |
| 基因名称 | WDR61 |
| Gene ID (NCBI) | 80349 |
| RRID | AB_11232419 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9GZS3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency (refer to UniProt). Catalog#22536-1-AP is a rabbit polyclonal antibody raised against the full-length of human WDR61. The MW of this protein is 33 kDa, and this antibody specially recognises the 33 kDa protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR61 antibody 22536-1-AP | Download protocol |
| IHC protocol for WDR61 antibody 22536-1-AP | Download protocol |
| IF protocol for WDR61 antibody 22536-1-AP | Download protocol |
| IP protocol for WDR61 antibody 22536-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv FOXA1 O-GlcNAcylation-mediated transcriptional switch governs metastasis capacity in breast cancer | ||
Mol Cancer Ther Targeting the synthetic lethal relationship between FOCAD and TUT7 represents a potential therapeutic opportunity for TUT4/7 small molecule inhibitors in cancer | ||
Cell Death Dis UBR5 targets tumor suppressor CDC73 proteolytically to promote aggressive breast cancer. | ||
FEBS J WDR61 ablation triggers R-loop accumulation and suppresses breast cancer progression |
