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WFDC1 Polyclonal antibody

WFDC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13204-1-AP

Print datasheet

Synonyms

Prostate stromal protein ps20, PS20, ps20 growth inhibitor, WFDC1

验证数据展示

  • WB analysis of HEK-293 using 13204-1-AP

    WB analysis of HEK-293 using 13204-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13204-1-AP (WFDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 13204-1-AP

    IHC staining of human breast cancer using 13204-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13204-1-AP (WFDC1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 13204-1-AP

    IHC staining of human breast cancer using 13204-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13204-1-AP (WFDC1 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13204-1-AP targets WFDC1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen WFDC1 fusion protein Ag3960 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WAP four-disulfide core domain 1
Synonyms Prostate stromal protein ps20, PS20, ps20 growth inhibitor, WFDC1
Calculated Molecular Weight 220 aa, 24 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC029159
Gene Symbol WFDC1
Gene ID (NCBI) 58189
RRIDAB_2215895
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HC57
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for WFDC1 antibody 13204-1-APDownload protocol
IHC protocol for WFDC1 antibody 13204-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols