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验证数据展示

  • WB analysis of HEK-293 using 13204-1-AP

    WB analysis of HEK-293 using 13204-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13204-1-AP (WFDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 13204-1-AP

    IHC staining of human breast cancer using 13204-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13204-1-AP (WFDC1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 13204-1-AP

    IHC staining of human breast cancer using 13204-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13204-1-AP (WFDC1 Antibody) at dilution of 1:50 (under 40x lens).

WFDC1 Polyclonal antibody

WFDC1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13204-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Prostate stromal protein ps20, PS20, ps20 growth inhibitor, WFDC1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13204-1-AP targets WFDC1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 WFDC1 fusion protein Ag3960 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WAP four-disulfide core domain 1
别名 Prostate stromal protein ps20, PS20, ps20 growth inhibitor, WFDC1
计算分子量 220 aa, 24 kDa
观测分子量 29 kDa
GenBank蛋白编号BC029159
基因名称 WFDC1
Gene ID (NCBI) 58189
RRIDAB_2215895
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HC57
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for WFDC1 antibody 13204-1-APDownload protocol
IHC protocol for WFDC1 antibody 13204-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols