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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 67291-1-Ig

    WB analysis using 67291-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67291-1-Ig (WFS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of A172 using 67291-1-Ig

    WB analysis of A172 using 67291-1-Ig

    A172 cells were subjected to SDS PAGE followed by western blot with 67291-1-Ig (WFS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67291-1-Ig

    WB analysis using 67291-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67291-1-Ig (WFS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

WFS1 Monoclonal antibody

WFS1 Monoclonal Antibody for WB, ELISA
Cat No. 67291-1-Ig

产品说明书

CloneNo. 4C2B5

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig

应用

WB, ELISA

别名

FLJ51211, WFRS, WFS, WFS1, Wolfram syndrome 1 (wolframin), WOLFRAMIN

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, SH-SY5Y cells, A172 cells, HT-29 cells, HEK-293 cells, C6 cells, U-251 cells, PC-12 cells, Neuro-2a cells, SW480 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy WFS1 Ready-To-Use IHC Kit. WFS1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67291-1-Ig targets WFS1 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat, pig
免疫原 WFS1 fusion protein Ag25735 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Wolfram syndrome 1 (wolframin)
别名 FLJ51211, WFRS, WFS, WFS1, Wolfram syndrome 1 (wolframin), WOLFRAMIN
计算分子量 890 aa, 100 kDa
观测分子量 100 kDa
GenBank蛋白编号BC030130
基因名称 WFS1
Gene ID (NCBI) 7466
RRIDAB_2882556
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO76024
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss.

实验方案

Product Specific Protocols
WB protocol for WFS1 antibody 67291-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols