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验证数据展示

  • FC experiment of HeLa using 83116-2-RR (same clone as 83116-2-PBS)

    FC experiment of HeLa using 83116-2-RR (same clone as 83116-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug NSD3 Recombinant antibody (83116-2-RR, Clone:230499C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83116-2-PBS in a different storage buffer formulation.

  • WB analysis using 83116-2-RR (same clone as 83116-2-PBS)

    WB analysis using 83116-2-RR (same clone as 83116-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83116-2-RR (WHSC1L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83116-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00189-4

    Sandwich ELISA standard curve of MP00189-4

    Sandwich ELISA standard curve of MP00189-4, Human NSD3 Recombinant Matched Antibody Pair - PBS only. 83116-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1894. 83116-5-PBS was HRP conjugated as the detection antibody. Range: 19.5-625 pg/mL.

  • Sample test of MP00189-4

    Sample test of MP00189-4

    The mean NSD3 concentration was determined to be 542.5 pg/mL in A549 cell extract based on a 1.0 mg/mL extract load, 565.1 pg/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load and 529.6 pg/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

  • IHC staining of human lung squamous cell carcinoma using 83116-2-RR (same clone as 83116-2-PBS)

    IHC staining of human lung squamous cell carcinoma using 83116-2-RR (same clone as 83116-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 83116-2-RR (NSD3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83116-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00189-2

    Cytometric bead array standard curve of MP00189-2

    Cytometric bead array standard curve of MP00189-2, WHSC1L1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83116-2-PBS. Detection antibody: 83116-4-PBS. Standard: Ag1894. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83116-2-RR

    Affinity and Kinetic Characterization of 83116-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83116-2-RR against Human NSD3 were performed. The affinity constant is below 1 pM.

NSD3 Recombinant antibody, PBS Only (Capture)

NSD3 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83116-2-PBS

产品说明书

CloneNo. 230499C2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

WHSC1L1, Protein whistle, pp14328, Nuclear SET domain-containing protein 3, Histone-lysine N-methyltransferase NSD3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83116-2-PBS targets NSD3 as part of a matched antibody pair:

MP00189-2: 83116-2-PBS capture and 83116-4-PBS detection (validated in Cytometric bead array)

MP00189-4: 83116-2-PBS capture and 83116-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 NSD3 fusion protein Ag1894 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Wolf-Hirschhorn syndrome candidate 1-like 1
别名 WHSC1L1, Protein whistle, pp14328, Nuclear SET domain-containing protein 3, Histone-lysine N-methyltransferase NSD3
计算分子量 1437 aa, 162 kDa
观测分子量 160-170 kDa, 68-72 kDa
GenBank蛋白编号BC012059
基因名称 WHSC1L1
Gene ID (NCBI) 54904
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BZ95
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WHSC1L1, also named as NSD3 and DC28, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is histone methyltransferase. WHSC1L1 is a potently transforming oncogenes based on the number of altered phenotypes expressed by the cells. It contains a PWWP-domain that is a methyl-lysine recognition motif involved in histone code modification and epigenetic regulation of gene expression. Knockdown of WHSC1L1 in 8p11-12-amplified breast cancer cells resulted in profound loss of growth and survival of these cells. (PMID: 20940404). This antibody recognize the long isoform (160-170 kDa) and short isoform (68-72 kDa).