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NSD3 Recombinant antibody

NSD3 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230499C2

Cat no : 83116-2-RR

Print datasheet

Synonyms

WHSC1L1, Protein whistle, pp14328, Nuclear SET domain-containing protein 3, Histone-lysine N-methyltransferase NSD3

验证数据展示

  • FC experiment of HeLa using 83116-2-RR

    FC experiment of HeLa using 83116-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug NSD3 Recombinant antibody (83116-2-RR, Clone:230499C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • WB analysis using 83116-2-RR

    WB analysis using 83116-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83116-2-RR (WHSC1L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung squamous cell carcinoma using 83116-2-RR

    IHC staining of human lung squamous cell carcinoma using 83116-2-RR

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 83116-2-RR (NSD3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83116-2-RR

    Affinity and Kinetic Characterization of 83116-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83116-2-RR against Human NSD3 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, K-562 cells
Positive IHC detected inhuman lung squamous cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83116-2-RR targets NSD3 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen NSD3 fusion protein Ag1894 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Wolf-Hirschhorn syndrome candidate 1-like 1
Synonyms WHSC1L1, Protein whistle, pp14328, Nuclear SET domain-containing protein 3, Histone-lysine N-methyltransferase NSD3
Calculated Molecular Weight 1437 aa, 162 kDa
Observed Molecular Weight 160-170 kDa, 68-72 kDa
GenBank Accession NumberBC012059
Gene Symbol WHSC1L1
Gene ID (NCBI) 54904
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BZ95
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WHSC1L1, also named as NSD3 and DC28, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is histone methyltransferase. WHSC1L1 is a potently transforming oncogenes based on the number of altered phenotypes expressed by the cells. It contains a PWWP-domain that is a methyl-lysine recognition motif involved in histone code modification and epigenetic regulation of gene expression. Knockdown of WHSC1L1 in 8p11-12-amplified breast cancer cells resulted in profound loss of growth and survival of these cells. (PMID: 20940404). This antibody recognize the long isoform (160-170 kDa) and short isoform (68-72 kDa).

实验方案

Product Specific Protocols
WB protocol for NSD3 antibody 83116-2-RRDownload protocol
IHC protocol for NSD3 antibody 83116-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols