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验证数据展示

  • WB analysis using 26406-1-AP

    WB analysis using 26406-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26406-1-AP (WISP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 26406-1-AP

    IHC staining of human colon cancer using 26406-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26406-1-AP (WISP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 26406-1-AP

    IHC staining of human colon cancer using 26406-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26406-1-AP (WISP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 26406-1-AP

    IF Staining of MCF-7 using 26406-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using WISP3 antibody (26406-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

WISP3 Polyclonal antibody

WISP3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26406-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

WNT1-inducible-signaling pathway protein 3, WISP-3, Cellular communication network factor 6, CCN6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inCaco-2 cells, COLO 320 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26406-1-AP targets WISP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen WISP3 fusion protein Ag23999 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WNT1 inducible signaling pathway protein 3
Synonyms WNT1-inducible-signaling pathway protein 3, WISP-3, Cellular communication network factor 6, CCN6
Observed Molecular Weight39-42 kDa
GenBank Accession NumberBC105941
Gene Symbol WISP3
Gene ID (NCBI) 8838
RRIDAB_3085867
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95389
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WISP3, also known as CCN6. Localized to the Mitochondria, and It is reported that CCN6 is located in the nucleus of breast cancer (PMID: 21262769). It is mainly expressed in adult kidney, testis and fetal kidney, and weakly expressed in placenta, ovary, prostate and small intestine (PMID: 9843955, PMID: 10471507). This gene is overexpressed in colon tumors, and it may be located downstream of WNT1 signaling pathway related to malignant transformation. Mutations of this gene are associated with progressive pseudorheumatoid dysplasia, an autosomal recessive skeletal disorder, indicating that the gene is essential for normal postnatal skeletal growth and cartilage homeostasis. Also it plays an important role in mitochondrial electron transfer and mitochondrial respiration, and may be important in bone growth and cartilage homeostasis after normal birth through its regulation of mitochondrial function (PMID: 27252383, PMID: 10471507). The calculated molecular weight of WISP3 is 39 kDa, and this antibody can recognize the 41 kDa isoform of WISP3.

实验方案

Product Specific Protocols
WB protocol for WISP3 antibody 26406-1-APDownload protocol
IHC protocol for WISP3 antibody 26406-1-APDownload protocol
IF protocol for WISP3 antibody 26406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols