• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68149-1-Ig (same clone as 68149-1-PBS)

    WB analysis using 68149-1-Ig (same clone as 68149-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68149-1-Ig (WNK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68149-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293T using 68149-1-Ig (same clone as 68149-1-PBS)

    FC experiment of HEK-293T using 68149-1-Ig (same clone as 68149-1-PBS)

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human WNK1 (68149-1-Ig, Clone:1B6A10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68149-1-PBS in a different storage buffer formulation.

WNK1 Monoclonal antibody, PBS Only

WNK1 Monoclonal Antibody for Indirect ELISA, WB
Cat No. 68149-1-PBS

产品说明书

CloneNo. 1B6A10

宿主/亚型

Mouse / IgG2a

种属反应性

rat, mouse, human

应用

Indirect ELISA, WB

别名

p65, KDP, HSN2, HSAN2, EC:2.7.11.1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy WNK1 Ready-To-Use IHC Kit. WNK1 primary antibody included.

产品信息

68149-1-PBS targets WNK1 in Indirect ELISA, WB applications and shows reactivity with rat, mouse, human samples.

经测试应用 Indirect ELISA, WB Application Description
经测试反应性 rat, mouse, human
免疫原 WNK1 fusion protein Ag28044 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 WNK lysine deficient protein kinase 1
别名 p65, KDP, HSN2, HSAN2, EC:2.7.11.1
计算分子量 251 kDa
观测分子量250 kDa
GenBank蛋白编号BC021121
基因名称 WNK1
Gene ID (NCBI) 65125
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H4A3
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WNK kinase is a unique serine-threonine kinase in which the lysine (K) residue in the catalytic domain, that is found in the majority of kinases is not conserved. In mammals, there are four WNK kinase family members (WNK1-4). WNK1 has 6 isoforms, such as long form WNK1 (L-WNK1, 250 kDa, strong expression in dorsal root ganglia and spinal cord) ; kidney-specific isoform (KS-WNK1, 207 kDa, lacks a kinase domain).KS-WNK1 cannot phosphorylate substrates and instead interacts with and modulates the actions of other WNKs via a carboxyl terminal HQ domain.