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WNK1 Monoclonal antibody, PBS Only

WNK1 Monoclonal Antibody for Indirect ELISA, WB

Host / Isotype

Mouse / IgG2a

Reactivity

rat, mouse, human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

1B6A10

Cat no : 68149-1-PBS

Print datasheet

Synonyms

p65, KDP, HSN2, HSAN2, EC:2.7.11.1

验证数据展示

  • WB analysis using 68149-1-Ig (same clone as 68149-1-PBS)

    WB analysis using 68149-1-Ig (same clone as 68149-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68149-1-Ig (WNK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68149-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293T using 68149-1-Ig (same clone as 68149-1-PBS)

    FC experiment of HEK-293T using 68149-1-Ig (same clone as 68149-1-PBS)

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human WNK1 (68149-1-Ig, Clone:1B6A10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68149-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy WNK1 Ready-To-Use IHC Kit. WNK1 primary antibody included.

产品信息

68149-1-PBS targets WNK1 in Indirect ELISA, WB applications and shows reactivity with rat, mouse, human samples.

Tested Applications Indirect ELISA, WB Application Description
Tested Reactivity rat, mouse, human
Immunogen WNK1 fusion protein Ag28044 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name WNK lysine deficient protein kinase 1
Synonyms p65, KDP, HSN2, HSAN2, EC:2.7.11.1
Calculated Molecular Weight 251 kDa
Observed Molecular Weight250 kDa
GenBank Accession NumberBC021121
Gene Symbol WNK1
Gene ID (NCBI) 65125
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H4A3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WNK kinase is a unique serine-threonine kinase in which the lysine (K) residue in the catalytic domain, that is found in the majority of kinases is not conserved. In mammals, there are four WNK kinase family members (WNK1-4). WNK1 has 6 isoforms, such as long form WNK1 (L-WNK1, 250 kDa, strong expression in dorsal root ganglia and spinal cord) ; kidney-specific isoform (KS-WNK1, 207 kDa, lacks a kinase domain).KS-WNK1 cannot phosphorylate substrates and instead interacts with and modulates the actions of other WNKs via a carboxyl terminal HQ domain.